Jun 26, 2024
Preparation of PEI for primary coating of MaxOne (neuronal cultures)
- 1Weizmann Institute
Protocol Citation: Yonatan Katz 2024. Preparation of PEI for primary coating of MaxOne (neuronal cultures). protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk8dy6l5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 25, 2024
Last Modified: June 26, 2024
Protocol Integer ID: 102349
Keywords: primary coating of maxone, cell adhesion on maxone chip, pei coating, preparation of pei, primary coating, laminin coating, maxone chip, coating, cell adhesion, polyethyleneimine, maxone, adhesion, pei, neuronal culture
Abstract
Polyethyleneimine (PEI) coating to enhance cell adhesion on MaxOne chips. PEI coating is followed by Laminin coating.
Protocol materials
Pierce™ 20X Borate BufferThermo FisherCatalog #28341
Poly(ethyleneimine) solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3143
Materials
Pierce™ 20X Borate BufferThermo FisherCatalog #28341
Poly(ethyleneimine) solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3143
0.07% PEI solution preparation - Primary coating
30m
Prepare 100 mL of 1X borate buffer:
5 mL 20X borate buffer
95 mL sterile deionized water
7% PEI stock solution:
1.4 mL 50% = PEI solution directly into a sterile beaker.
8.6 mL 1X borate buffer into the sterile beaker.
Mix 00:30:00 using a magnetic stirrer until fully dissolved
(can be stored in 0.5 mL aliquots at -20°C for 1 month).
Where is it sterilized? -
30m
final 0.07% PEI solution
0.5 mL 7% PEI solution in
49.5 mL 1X borate buffer fresh
filter through a 0.22 μm filter unit before use!