Jul 19, 2022

Public workspace Preparation of M9 worm buffer V.2

DOI
https://dx.doi.org/10.17504/protocols.io.x54v9yo6pg3e/v2
  • Alfonso Pérez Escudero1,
  • Alid Al-Asmar1,
  • Gabriel Madirolas1
  • 1CNRS, Université Paul Sabatier (Toulouse III)
  • Perez-Escudero Lab
Alfonso Pérez Escudero
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DOI: https://dx.doi.org/10.17504/protocols.io.x54v9yo6pg3e/v2
Protocol Citation: Alfonso Pérez Escudero, Alid Al-Asmar, Gabriel Madirolas 2022. Preparation of M9 worm buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9yo6pg3e/v2Version created by APE Lab
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: July 04, 2022
Last Modified: July 19, 2022
Protocol Integer ID: 65912
Keywords: preparation of m9 worm buffer preparation, m9 worm buffer preparation, m9 worm buffer, caenorhabditis elegans in liquid, caenorhabditis elegan, preparation
Abstract
Preparation of M9 worm buffer, used to maintain Caenorhabditis elegans in liquid for short periods of time. Protocol taken from the WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
Materials
Our references (also check our magnesium sulfate solution protocol):
Monopotassium phosphate (KH2PO4): ReagentPotassium phosphate monobasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #795488-500G
Disodium phosphate dihydrate (Na2HPO4.2H2O): ReagentSodium phosphate dibasic dihydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #71643-250G
Sodium chloride (NaCl): ReagentSodium chlorideMerck MilliporeSigma (Sigma-Aldrich)Catalog #S5886-1KG

Troubleshooting
Add Amount3 g ±0.03 of monopotassium phosphate (KH2PO4) in a 1L clean bottle.

Add Amount7.52 g ±0.05 of disodium phosphate dihydrate (Na2HPO4.2H2O).

Add Amount5 g ±0.05 of sodium chloride (NaCl).

Add Amount1 L of milliQ water.

Autoclave.
Add Amount1 mL of magnesium sulfate solution. Our protocol for making this solution can be found here:

Protocol
NAME

Preparation of 1M magnesium sulfate solution (MgSO4)

CREATED BY
APE Lab