Oct 11, 2022

Public workspacePreparation of frozen nuclei for single-nucleus RNA seq V.2

DOI
https://dx.doi.org/10.17504/protocols.io.eq2lyrdpgx9k/v2
  • Kimberly Siletti1,
  • Sten Linnarsson1
  • 1Karolinska Institutet
  • Human Cell Atlas Method Development Community
  • BICCN / BICAN
Kimberly Siletti
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DOI: https://dx.doi.org/10.17504/protocols.io.eq2lyrdpgx9k/v2
Protocol Citation: Kimberly Siletti, Sten Linnarsson 2022. Preparation of frozen nuclei for single-nucleus RNA seq. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyrdpgx9k/v2Version created by Kimberly Siletti
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 11, 2022
Last Modified: October 11, 2022
Protocol Integer ID: 71166
Keywords: nucleus rna seq this protocol, nucleus rna seq, preparation of frozen nuclei, sorted frozen nuclei, frozen nuclei, preparation of nuclei, nucleus rna, 10x genomics chromium system, rna, nuclei
Abstract
This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted frozen nuclei. 
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