Oct 18, 2021
Preparation of BG-11 media Agar
- 1Indian Institute od Science Education and Research, Pune
- iGEM IISER Pune India 2021
Protocol Citation: Ashwinuday 2021. Preparation of BG-11 media Agar . protocols.io https://dx.doi.org/10.17504/protocols.io.by6rpzd6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: October 18, 2021
Last Modified: October 18, 2021
Protocol Integer ID: 54193
Abstract
This is for making solid media of BG-11
To make BG-11 Agar plates, add Agar-Agar Type-1 equivalent to 1.5% (w/v) of liquid media and autoclave.
Then before it solidifies (when the flask is bearable to touch), add the required antibiotics to the media.
Pour ~30-40 ml into plates soon after.
Keep the plates opened in the hood.
Once it solidifies in the plates then close those and keep the plates inverted into a cold storage.