Oct 18, 2021
Preparation of BG-11 liquid media
- 1Indian Institute of Science Education and Research, Pune
- iGEM IISER Pune India 2021
Protocol Citation: Ashwinuday 2021. Preparation of BG-11 liquid media . protocols.io https://dx.doi.org/10.17504/protocols.io.by6qpzdw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: October 18, 2021
Last Modified: October 18, 2021
Protocol Integer ID: 54192
Abstract
This is for making liquid media of BG-11.
Take the medium in powdered form and mix it with doubly distilled water to just 1/10 th of the total volume.
Add a common stock of micronutrients made in ddH2O and mix well.
Make up the volume to the total and mixed well.
Adjust the pH on a calibrated pH meter using 1N HCl and /or 1N NaOH. The media was mixed using a magnetic stirrer continuously to homogenize the pH and to prevent precipitation of certain constituents.
This mix was autoclaved.