May 17, 2024
Preparation of acid-washed glass coverslips for immunofluorescence microscopy
- Jenna Ekstrom1
- 1The University of Alabama at Birmingham
Protocol Citation: Jenna Ekstrom 2024. Preparation of acid-washed glass coverslips for immunofluorescence microscopy. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr847blmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 17, 2024
Last Modified: May 17, 2024
Protocol Integer ID: 99982
Keywords: glass coverslips for immunofluorescence microscopy rizzardi lab, immunofluorescence microscopy rizzardi lab, washed glass coverslip, preparation of acid, immunofluorescence
Abstract
Rizzardi Lab (Adapted from Schwer Lab- UCSF)
Troubleshooting
Add coverslips to 10cm culture dish
Add ~20 mL of 1 N HCl; swirl on orbital shaker for 2 h at RT.Room temperature
2h
Remove acid, rinse with 2 x 25 mL PBS until pH is at least 6.0.
Rinse 1 x 25 mL water.
Add ~20 mL of 70% EtOH; swirl on orbital shaker for 10 min, RT.Room temperature
10m
Remove ethanol, transfer coverslips to Whatman paper. Let dry in TC hood.
Sterilize coverslips before use
Option 1:
Transfer to glass beaker, cover with aluminum foil and autoclave (dry cycle, 30 min sterilization time)
Option 2:
Transfer to new sterile 10cm culture dish and turn on UV. Leave in hood until ready for use.