Jul 19, 2022
Preparation of 1M magnesium sulfate solution (MgSO4)
- Alid Al-Asmar1,
- Alfonso Pérez Escudero1,
- gabrielmadirolas 1
- 1CNRS, Université Paul Sabatier (Toulouse III)
- Perez-Escudero Lab
Protocol Citation: Alid Al-Asmar, Alfonso Pérez Escudero, gabrielmadirolas 2022. Preparation of 1M magnesium sulfate solution (MgSO4). protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1nmbygr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: June 11, 2022
Last Modified: July 19, 2022
Protocol Integer ID: 64369
Keywords: magnesium sulfate solution, preparation of worm media, mgso4, worm media, nematode growth medium, preparation, ngm, original source of the protocol
Abstract
Original source of the protocol: WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
This protocol is for making 1M magnesium sulfate solution, which is used in the preparation of worm media, like NGM (Nematode Growth Medium).
Materials
Magnesium sulfate: Magnesium Suflate HeptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M5921-500G
Filter: Rapid-Flow Nalgene 0.2µm aPES membrane, ref: 564-0020
Vaccum pump: Fisher Brand, ref: FB70155
Troubleshooting
Weigh 12.3 g ±0.1 of magnesium sulfate heptahydrate (MgSO4·7H2O), and add it to a 100 mL measuring cylinder.
Add ~40 mL of milliQ water.
Add a clean stirring magnet, and leave it on a stirrer until all crystals are dissolved.
Remove the stirring magnet: To prevent touching the solution, use another magnet from the outside of the measuring cylinder to slide the stirring magnet out of the cylinder.
Fill up to 50 mL with milliQ water
Filter-sterilize.
Store at 4°C.
We usually use it without problem for up to at least a year after preparation.