Dec 12, 2016
Pre-stain protocol for GreenGlo™ Safe DNA Dye, 20,000X in Water
- Denville Scientific
- Denville Scientific, Inc.
External link: https://www.denvillescientific.com/products/greenglo%E2%84%A2-safe-dna-dye--20-000x-in-water--500%C2%B5l
Protocol Citation: Denville Scientific 2016. Pre-stain protocol for GreenGlo™ Safe DNA Dye, 20,000X in Water. protocols.io https://dx.doi.org/10.17504/protocols.io.gp6bvre
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: December 09, 2016
Last Modified: March 07, 2018
Protocol Integer ID: 4574
Abstract
GreenGlo ™, 20,000X in Water, is a non-carcinogenic and non-toxic alternative to Ethidium bromide used for the detection of nucleic acids in agarose gels. It is as sensitive as Ethidium bromide. There is no toxic DMSO as GreenGlo ™ is supplied in water.
GreenGlo ™ has fluorescence excitation maxima at 295 nm and 490 nm. The fluorescence emission maxima is similar to EtBr when bound to DNA – at 530 nm.
Materials
MATERIALS
GreenGlo™ Safe DNA Dye, 20,000X in Water, 500µlDenville Scientific Inc.Catalog #CA3600
PRE-STAIN
PRE-STAIN
Add GreenGlo ™ to melted agarose when the agarose has cooled to 50 to 60ºC.
Note
Use 4-6 µl per 100 ml of agarose or 2-3 µl per 50 ml of agarose
Note
IMPORTANT : GreenGlo ™ is supplied in 20,000X concentration in water
DETECTION
DETECTION
Detect bands under UV illumination (yellow or green gelatin- or cellophane filters is recommended for clearer bands) or non-UV LED illuminators such as Blue Light LED illumination.