Feb 21, 2023

Potassium phosphate buffer (0.9 M, pH 7.7) V.2

This protocol is a draft, published without a DOI.
Potassium phosphate buffer (0.9 M, pH 7.7)
  • 1University of Oslo
Andreas Sagen
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Protocol Citation: Andreas Sagen 2023. Potassium phosphate buffer (0.9 M, pH 7.7). protocols.io https://dx.doi.org/Version created by Andreas Sagen
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: February 20, 2023
Last Modified: February 21, 2023
Protocol  Integer ID: 77334
Keywords: potassium phosphate buffer, buffer, protein, buffering capacity, ph, sodium
Abstract
Potassium phosphate buffers are buffers with a buffering capacity between 5.8 and 8.0, where it is possible to adjust the pH and buffer strength with different ratios of Sodium phosphate monobasic and Sodium phosphate dibasic.
Materials
Scale
Magnetic stirrer
Filtering device
Protocol materials
Potassium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3786
Potassium phosphate monobasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P5379
500 mL Phosphate buffer (0.9 M, pH 7.7)
5m
Fill a sterile 500 mL bottle with 400 mL distilled water
Measure 62.705 g Potassium phosphate dibasic and 11.568 g Potassium phosphate monobasic

Materials:
Potassium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3786
Potassium phosphate monobasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P5379
Add dry ingredients and mix for 00:05:00
5m
Fill to 500 mL with distilled water
Filter sterilize through a 0.2 µm filter and store refrigerated (4 °C )