Oct 11, 2022
Post-Surgical Dissection of Fallopian Tubes
- Stephen Fisher1,
- Marielena Grijalva1,
- Rong Guo1,
- sarahjoh 1,
- Hieu Nguyen1,
- John Renz2,
- Jean G Rosario1,
- Steven Rudich2,
- Brian Gregory1,
- Junhyong Kim1,
- Kate O'Neill1
- 1University of Pennsylvania;
- 2Gift of Life Donor Program
Protocol Citation: Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh , Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill 2022. Post-Surgical Dissection of Fallopian Tubes. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn75eqv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 05, 2022
Last Modified: October 11, 2022
Protocol Integer ID: 68275
Keywords: dissection of the fallopian tube, fallopian tube, fallopian tube the muscule layer, dissection, dissection pin, tube, preparation for 10x visium, muscule layer, 10x visium, 10x multiomic, pathology review, pathology, muscular layer, inner mucosa
Funders Acknowledgements:
NIH
Grant ID: U54HD104392
Abstract
This protocol describes dissection of the Fallopian tubes in preparation for 10X Visium, 10X Multiomics, pathology review, and biobanking. The Fallopian tubes have an inner mucosa surrounded by a muscular layer that is coated by serosa. When sectioning the Fallopian tube the muscule layer may retract or protrude and distort orientation. This can be addressed by using a stabilizing hand or dissection pins.
Materials
- Ice
- Clean cutting surface
- Disposable scalpels
- Kimwipes
- Disposble rulers (with centimeter measurements)
- Ice bucket
- Biohazard bags and container
- Nuclease-free Water
- DMEM/F-12Thermo FisherCatalog #11320033
- RPMI 1640 MediumThermo FisherCatalog #11875085
- MACS® Tissue Storage SolutionMiltenyi BiotecCatalog #130-100-008
- DPBS (10X), no calcium, no magnesium Thermo Fisher ScientificCatalog #14200075 - dilute to 1X with nuclease-free water
- Marking dyeCancer DiagnosticsCatalog #03000P
Equipment
VWR® Pour-Boat Weighing Dishes
NAME
flat-bottomed dish
TYPE
VWR International
BRAND
10803-166
SKU
Troubleshooting
Before start
Dilute phosphate buffered saline (Gibco; 14200-075) to 1X with nuclease-free water.
After removing uterus and Fallopian tubes from ice, dry each Fallopian tube and use marking dye to label the superior edge (12 o’clock), inferior edge (6 o’clock), anterior surface (3 o’clock) and posterior surface (9 o’clock).
Measure the Fallopian tube from cornua to fimbriated end.
Measurement of Fallopian Tube. Left Fallopian tube is displayed from isthmus (left) to fimbriated end (right). Black line made with marking ink at 3 o’clock and red line made at 6 o’clock where the Fallopian tube attaches to the mesosalpinx.
Using a disposable scalpel, divide one Fallopian tube at the cornua and excise mesosalpinx.
Weigh Fallopian tube in a disposable weigh boat on ice.
Place on ice the uterus with the remaining contralateral Fallopian tube.
Remove the first Fallopian tube from ice and place on a cutting surface.
Divide Fallopian tube into four sections: isthmus, ampulla, infundibulum, and fimbriae.
Working one section at a time, slice tissue into four sections.
Using disposable weigh boats, weigh each tissue piece.
Tissue can be processed with protocols OCT-Embedded Tissue Preparation, Tissue Fixation Preparation, or Snap-Frozen Tissue Preparation depending on the desired downstream processing.
Remove the uterus with the remaining contralateral Fallopian tube from ice and repeat above steps.