Dec 12, 2016
Post-stain protocol for GreenGlo™ Safe DNA Dye, 20,000X in Water
This protocol is a draft, published without a DOI.
- Denville Scientific
- Denville Scientific, Inc.
External link: https://www.denvillescientific.com/products/greenglo%E2%84%A2-safe-dna-dye--20-000x-in-water--500%C2%B5l
Protocol Citation: Denville Scientific 2016. Post-stain protocol for GreenGlo™ Safe DNA Dye, 20,000X in Water. protocols.io https://dx.doi.org/10.17504/protocols.io.gqcbvsw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: December 09, 2016
Last Modified: February 03, 2018
Protocol Integer ID: 4580
Abstract
GreenGlo ™, 20,000X in Water, is a non-carcinogenic and non-toxic alternative to Ethidium bromide used for the detection of nucleic acids in agarose gels. It is as sensitive as Ethidium bromide. There is no toxic DMSO as GreenGlo ™ is supplied in water.
GreenGlo ™ has fluorescence excitation maxima at 295 nm and 490 nm. The fluorescence emission maxima is similar to EtBr when bound to DNA – at 530 nm.
Materials
MATERIALS
GreenGlo™ Safe DNA Dye, 20,000X in Water, 500µlDenville Scientific Inc.Catalog #CA3600
POST-STAIN
POST-STAIN
Use 10 to 15 µl per 100 ml of staining solution. Same as when using ethidium bromide.
Note
For an average gel thickness of about 7 mm, stain 30 minutes.
00:03:00
Destain of 30 minutes in water.
00:30:00
Note
Protect gel and staining solution from light with aluminum foil or place in dark.
DETECTION
DETECTION
Detect bands under UV illumination (yellow or green gelatin- or cellophane filters is recommended for clearer bands) or non-UV LED illuminators such as Blue Light LED illumination.