Jun 03, 2020
Version 5
Post Patch Clamp Slice Fixation V.5
- Allen Institute for Brain Science1
- 1Allen Institute
- BICCN / BICAN
- Allen Institute for Brain Science
Protocol Citation: Allen Institute for Brain Science 2020. Post Patch Clamp Slice Fixation. protocols.io https://dx.doi.org/10.17504/protocols.io.bg5tjy6n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 03, 2020
Last Modified: March 16, 2021
Protocol Integer ID: 37779
Keywords: Patch Clamp, fixation, electrophysiology, biocytin, PF0289,
Abstract
This protocol describes the process to fix recorded/filled cells from mouse and human brain slices with 4% PFA/2.5% Glutaraldehyde in PBS for future histochemical processing.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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