Jun 11, 2019

Public workspacePoly-d lysine coating slides

DOI
dx.doi.org/10.17504/protocols.io.32cgqaw
  • 1Regev Lab
Grace Burgin
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.32cgqaw
Protocol Citation: Grace Burgin 2019. Poly-d lysine coating slides. protocols.io https://dx.doi.org/10.17504/protocols.io.32cgqaw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 11, 2019
Last Modified: June 11, 2019
Protocol Integer ID: 24356
Abstract
coating standard blank slides with poly-d lysine to increase adhesion of tissue
Make .3mg/ml in 0.1M borate buffer [ → add 16.67 ml 0.1M borate to 5mg bottle of poly-D lysine lyophylized powder]
coat the slides once by dipping
Dry
Rinse twice with dH20
Dry