Jan 24, 2025
Version 2
Pole Test V.2
- daniel.dautan daniel1,2,
- Per Svenningsson1,2
- 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2025. Pole Test. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2qqj3l1y/v2Version created by Jacquelyn Haytayan
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 05, 2024
Last Modified: January 24, 2025
Protocol Integer ID: 118992
Keywords: ASAPCRN, behavior, mouse, Parkinson's, motor impairmen
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Test for motor ability in mice
Materials
50cm long, 1 cm diameter, wooden pole placed perpendicularly to small stand
Set Up
Set Up
Place the wooden, 50 cm long, 1cm in diameter the home cage to be tested, and make sure it stands upright on stand.
Set up recording camera to record the trials.
Take measurements of mice body weight.
Clean pole completely with ethanol.
Protocol
Protocol
Habituate the mice to the pole one time before testing by carefully placing the mouse on the top of the pole facing downwards. Allow the mouse to travel down and reach the home cage.
After habituation, test the mouse by again placing them on the top of the pole facing downwards. Allow the mouse to travel down and reach the home cage. There is no time cut-off.
Record the time that the mice took to descend the first half, the second half, and the total pole.
Test each mouse 5 times rotating between other mice in the cage.
Be sure to clean the pole completely with ethanol between each cage to prevent olfactory bias.