Dec 01, 2023
- 1Massachusetts General Hospital, ASAP
Protocol Citation: Pranay Srivastava 2023. Pole test. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwdq5dlmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 30, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 91662
Keywords: ASAPCRN, pole test pole test, motor dysfunction in rodent, assessing motor dysfunction, behavior test, test, rodent
Abstract
Pole test is a behavior test for assessing motor dysfunction in rodents.
Set up a vertical pole (1 cm diameter, 50 cm height) in a controlled testing
environment.
Allow the animals to acclimate to the testing environment for one hour before starting the test.
Mice were trained on the pole one day before the start of the experiment for 120s. Time taken by the mice to turn (T turn) and time taken to climb down (T descent) the pole were recorded (Matsuura et al. 1997; Sun et al. 2021).