Oct 21, 2020
PLASMA- 01 - Plasma separation from human whole blood
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Protocol Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. PLASMA- 01 - Plasma separation from human whole blood. protocols.io https://dx.doi.org/10.17504/protocols.io.bmhyk37w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 18, 2020
Last Modified: October 21, 2020
Protocol Integer ID: 42264
Materials
Plastic disposables
Before start
In order to prevent blood clotting during blood sampling, the addition of an anticoagulant (EDTA, sodium citrate or heparin) in the tubes is needed. Check for the presence of an anticoagulant in vacuum-sealed tubes used for blood sampling.
Put whole blood (at least 1.5 mL ) into a 5 mL tube.
Centrifuge at 1400 x g, Room temperature, 00:05:00
Equipment
Allegra AVANTI 30
NAME
Centrifuge
TYPE
Beckman Coulter
BRAND
Beckman Italy
SKU
Carefully collect the supernatant (plasma). Do not touch the red pellet on the bottom.
Note
≤ 85% of plasma should be retrieved to avoid contamination by the underlying cell pellet.
Store plasma at -80°C until use. Defrost only once.
EXPECTED RESULTS
Expected result
Expected plasma yield is approximately 50% of whole blood volume.