Jan 06, 2020

Public workspacePI staining of Arabidopsis seedling

DOI
dx.doi.org/10.17504/protocols.io.pejdjcn
  • 1Boyce Thompson Institute
  • Salt Lab KAUST
Magdalena M Julkowska
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DOI: dx.doi.org/10.17504/protocols.io.pejdjcn
Protocol Citation: Magdalena M Julkowska 2020. PI staining of Arabidopsis seedling. protocols.io https://dx.doi.org/10.17504/protocols.io.pejdjcn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 12, 2018
Last Modified: January 06, 2020
Protocol Integer ID: 11435
transfer the seedlings from agar plate into a solution containing the nutrients as well as 5-10 µg/mL of propidium iodide. Incubate for 20 to 30 min.
transfer the seedlings from agar plate into a solution containing the nutrients as well as 5-10 µg/mL of propidium iodide. Incubate for 20 to 30 min.

Examine the red staining under (confocal) microscope
Examine the red staining under (confocal) microscope