Sep 22, 2017

Public workspacePhytoplankton Protein Extraction with MP Biomedicals FastPrep bead beater

DOI
dx.doi.org/10.17504/protocols.io.jwncpde
  • 1https://sites.google.com/site/campbellphytoplankton/home
Natalie Donaher
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DOI: dx.doi.org/10.17504/protocols.io.jwncpde
Protocol Citation: Doug Campbell, Natalie Donaher 2017. Phytoplankton Protein Extraction with MP Biomedicals FastPrep bead beater. protocols.io https://dx.doi.org/10.17504/protocols.io.jwncpde
Manuscript citation:
Diatoms: PLoS ONE 8(1): e55562. doi:10.1371/journal.pone.0055562
Prasinophytes: https://link.springer.com/article/10.1007/s11120-016-0310-6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: September 15, 2017
Last Modified: January 17, 2018
Protocol Integer ID: 7854
Keywords: protein, phytoplankton, algae, FastPrep, bead beater
Abstract
A total protein extraction used by the Campbell Lab for phytoplankton cell pellets and GFF or polycarbonate filters. 
Attachments
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CampbellSOP18.FastPr...
156KB
Materials
MATERIALS
ReagentFastPrep Matrix D tubes 2 mLMP BiomedicalsCatalog #116913050
ReagentBeadBug 1.5 mm zirconium 2 mLMerck MilliporeSigma (Sigma-Aldrich)Catalog #Z763799-50EA