Jul 08, 2024

Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits.

  • 1Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland 20815, USA;
  • 3Department of Neurosurgery, Weill Cornell Medicine, New York, NY 10065;
  • 4Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon, 14662, Korea;
  • 5Departments of Neuroscience and Cell Biology, Howard Hughes Medical Institute, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06520, USA
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Collection CitationAlexandros C Kokotos, ama , Santiago Unda, Myung Soo Ko, Daehun Park, David Eliezer, Michael G. Kaplitt, Pietro De Camilli, Tim Ryan 2024. Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits.. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl495nzgo5/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 08, 2024
Last Modified: July 08, 2024
Collection  Integer ID: 103017
Keywords: ASAPCRN, phosphoglycerate kinase, central leverage point in parkinson, neuronal metabolic deficit, parkinson
Funders Acknowledgements:
ASAP
Grant ID: 000580
ASAP
Grant ID: 020608
Abstract
The current collection contains all experimental protocols associated with the study "Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits".
Files
Protocol
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Immunofluorescence for adherent cells
Version 1
Created by
Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
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Fast rodent genotyping
Version 1
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Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
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Primary hippocampal and cortical neuronal culture and transfection
Version 1
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Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
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Protocol for Neuronal Live-imaging of primary cultures
Version 1
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Image of Camila Pulido, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
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AAV Craniotomy
Version 1
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Image of Eileen Ruth Torres, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
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6-OHDA lesion in medial forebrain bundle
Version 1
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Image of Eileen Ruth Torres, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
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Apomorphine-induced rotations
Version 1
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Image of Eileen Ruth Torres, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
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Immunohistochemistry for brain sections
Version 1
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Image of Eileen Ruth Torres, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
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Genetic expression suppressor screen
Version 1
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Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
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vGlutI-pH imaging experiment analysis
Version 1
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Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
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Synapto-iATPSnFR2-miRFP670nano3 analysis
Version 1
Created by
Image of Alexandros C Kokotos, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine