Jul 09, 2024

Public workspacePhosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits.

  • 1Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland 20815, USA;
  • 3Department of Neurosurgery, Weill Cornell Medicine, New York, NY 10065;
  • 4Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon, 14662, Korea;
  • 5Departments of Neuroscience and Cell Biology, Howard Hughes Medical Institute, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06520, USA
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Collection CitationAlexandros C Kokotos, ama, Santiago Unda, Myung Soo Ko, Daehun Park, David Eliezer, Michael G. Kaplitt, Pietro De Camilli, Tim Ryan 2024. Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits.. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl495nzgo5/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 09, 2024
Last Modified: July 09, 2024
Collection Integer ID: 103017
Keywords: ASAPCRN
Funders Acknowledgements:
ASAP
Grant ID: 000580
ASAP
Grant ID: 020608
Abstract
The current collection contains all experimental protocols associated with the study "Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits".
Files
Protocol
Icon representing the file Immunofluorescence for adherent cells
Name
Immunofluorescence for adherent cells
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Icon representing the file Fast rodent genotyping
Name
Fast rodent genotyping
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Icon representing the file Primary hippocampal and cortical neuronal culture and transfection
Name
Primary hippocampal and cortical neuronal culture and transfection
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Icon representing the file Protocol for Neuronal Live-imaging of primary cultures
Name
Protocol for Neuronal Live-imaging of primary cultures
Version 1
, Weill Cornell Medicine
Camila PulidoWeill Cornell Medicine
Protocol
Icon representing the file AAV Craniotomy
Name
AAV Craniotomy
Version 1
, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
Protocol
Icon representing the file 6-OHDA lesion in medial forebrain bundle
Name
6-OHDA lesion in medial forebrain bundle
Version 1
, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
Protocol
Icon representing the file Apomorphine-induced rotations
Name
Apomorphine-induced rotations
Version 1
, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
Protocol
Icon representing the file Immunohistochemistry for brain sections
Name
Immunohistochemistry for brain sections
Version 1
, Weill Cornell Medicine
Eileen Ruth TorresWeill Cornell Medicine
Protocol
Icon representing the file Genetic expression suppressor screen
Name
Genetic expression suppressor screen
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Icon representing the file vGlutI-pH imaging experiment analysis
Name
vGlutI-pH imaging experiment analysis
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine
Protocol
Icon representing the file Synapto-iATPSnFR2-miRFP670nano3 analysis
Name
Synapto-iATPSnFR2-miRFP670nano3 analysis
Version 1
, Weill Cornell Medicine
Alexandros C KokotosWeill Cornell Medicine