Aug 29, 2025
Phosphate buffered saline (PBS)
- isabel.dilandro 1
- 1Claremont McKenna College
- Isabel DiLandro
Protocol Citation: isabel.dilandro 2025. Phosphate buffered saline (PBS). protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvjen7wgk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 28, 2025
Last Modified: August 29, 2025
Protocol Integer ID: 225739
Keywords: phosphate, saline, pb
Abstract
PBS
Guidelines
Makes 1L of PBS
Materials
Scale
Weigh boat
1L beaker
Distilled water (dH2O)
Sodium chloride (NaCl)
Potassium phosphate monobasic
Sodium phosphate dibasic
Hot plate
Stir bar
1L bottle
Troubleshooting
Safety warnings
When heating the mixture on the hot plate, make sure to not turn on the heat until the glass beaker is on the plate. Hot plate + cold glass = a bad time!
Directions
Fill a 1L beaker with 800mL of dH2O.
Weigh out 9g of NaCl, 3.8g of monobasic, and 10.2g of dibasic. Add these measurements to the beaker.
Add dH2O until the final volume of the solution reaches 1L.
Place beaker onto the hot plate and heat the solution up to 60°C.
Add stir bar and stir the solution until clear. Pour into a bottle and keep refrigerated.