Jun 02, 2020

Public workspacePhosphate Buffered Saline (PBS)

DOI
https://dx.doi.org/10.17504/protocols.io.bfypjpvn
Phosphate Buffered Saline (PBS)
  • Neilier Junior1
  • 1University of Manitoba
Neilier Junior
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DOI: https://dx.doi.org/10.17504/protocols.io.bfypjpvn
Protocol Citation: Neilier Junior 2020. Phosphate Buffered Saline (PBS). protocols.io https://dx.doi.org/10.17504/protocols.io.bfypjpvn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 04, 2020
Last Modified: June 02, 2020
Protocol Integer ID: 36591
Keywords: pH, Henderson-Hasselback, Biochemistry, Molecular Biology, phosphate buffered saline, buffer solution, buffered system, buffer, appropriate choice of the buffer, changes in ph, ph, biological condition, critical salt, essential nutrients for cell, adding powerful acid, essential nutrient, powerful acid, enzymatic reaction,
Abstract
A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
Materials
  • Distilled Water
  • pH Meter (sensitive)
  • NaCl
  • Potassium Phosphate Buffer
  • Na2HPO4
  • KH2PO4
Troubleshooting
Safety warnings
Wear personal protective equipment: gloves, lab coat and mask.
Before start
Organize your workspace.

Make sure all solutions and equipment are available.
Phosphate Buffered Saline (PBS)

Note
Ph7.4
150 mM NaCl
10 mM Potassium Phosphate buffer

Prepare Amount1 L PBS by dissolving Amount8.7 g NaCl ,Amount1.82 g K2HPO4 • 3H2O and Amount0.23 g KH2PO4 in Amount1 L distilled water .

A variation of PBS can also be prepared as follows:
Concentration137 millimolar (mM) NaCl
Concentration2.7 millimolar (mM) KCl
Concentration10 millimolar (mM) Na2HPO4
Concentration1.76 millimolar (mM) KH2PO4

Adjust the pH before use.