Oct 04, 2020

Public workspacepGEM-T连接

This protocol is a draft, published without a DOI.
  • 1西南大学
  • Zhang Xue
张 雪
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Protocol Citation: 张 雪 2020. pGEM-T连接. protocols.io https://protocols.io/view/pgem-t-bmzjk74n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 04, 2020
Last Modified: October 04, 2020
Protocol Integer ID: 42763
TemperatureOn ice
2× ligation buffer（用前振荡混匀）5µl
PCR回收产物3µl
pGEM-T Vector1µl
T4 ligase1µl


室温1h（20°C左右，16°C最佳）Temperature16 °C Duration01:00:00

随后4°C过夜Temperature4 °C DurationOvernight