This protocol describes the expression and purification procedures used in the Balog lab at the University of New England for producing recombinant elastin-like polymers (ELPs) in E. coli. It has been adapted from protocols used in the laboratory of Jennifer S. Martinez at Los Alamos National Laboratory, where it was originally developed based on the method of Hassouneh, Wafa et al. “Elastin-like polypeptides as a purification tag for recombinant proteins” Current protocols in protein science vol. Chapter 6 (2010): Unit 6.11.