May 16, 2020

Public workspacePCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515) V.1

DOI
dx.doi.org/10.17504/protocols.io.7sehnbe
  • 1New England Biolabs
  • New England Biolabs (NEB)
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DOI: dx.doi.org/10.17504/protocols.io.7sehnbe
External link: https://www.neb.com/protocols/2019/07/02/pcr-using-q5u-hot-start-high-fidelity-dna-polymerase-neb-m0515
Collection Citation: New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515). protocols.io https://dx.doi.org/10.17504/protocols.io.7sehnbe
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 28, 2019
Last Modified: March 30, 2021
Collection Integer ID: 28198
Keywords: Q5U
Abstract
Please note that protocols with Q5U Hot Start High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended in the protocols should be used for optimal performance.
Materials
MATERIALS
ReagentQ5U® Hot Start High-Fidelity DNA PolymeraseNew England BiolabsCatalog #M0515
Safety warnings
Please see SDS (Safety Data Sheet) for hazards and safety warnings.
Files
Protocol
Icon representing the file PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention
Name
PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention
Version 1
, New England Biolabs
New England BiolabsNew England Biolabs
Protocol
Icon representing the file PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): Amplification of bisulfite-converted, deaminated, or damaged DNA (Including FFPE DNA)
Name
PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): Amplification of bisulfite-converted, deaminated, or damaged DNA (Including FFPE DNA)
Version 1
, New England Biolabs
New England BiolabsNew England Biolabs