Partial sequences of mtDNA Control Region (CR) were amplified using the universal primers Thr-L15926 (5\u00b4-CAATTCCCCGGTCTTGTAAACC-3\u00b4), located in the neighboring tRNA-pro gene and DL-H16340 (5\u00b4-CCTGAAGTAGGAACCAGATG-3\u00b4) (Vil\u00e1 el al. 1999). Amplification of the double-stranded product was performed in 25 ul volume of PCR mix containing 1.25 U of Taq DNA polymerase, 2.5 ul of 10 x Taq polymerase buffer, 3\u00a0mM of MgCl2, 0,16 mM of dNTP\u00b4s and 5 pmol of each primer. The amplification was performed in a Biometra T Personal thermocycler. The thermal profile consisted of an initial denaturation at 94 \u00baC for 5 min, followed by 35 cycles of 94 \u00baC for 30 s, 56 \u00baC for 30 s and 72 \u00baC\u00a0 for 45 s with a final extension step of 72\u00baC for 8 min.