Aug 31, 2025

PCR & Library for Invertebrate Metabarcoding on MinION 

This  protocol  is a draft, published without a DOI.
  • Vid Švara1
  • 1FH Kärnten gGmbH
Vid Švara
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Protocol Citation: Vid Švara 2025. PCR & Library for Invertebrate Metabarcoding on MinION . protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: August 29, 2025
Last Modified: August 31, 2025
Protocol  Integer ID: 225829
Keywords: library for invertebrate metabarcoding, library preparation for invertebrate metabarcoding, invertebrate metabarcoding, minion platform, minion, pcr, protocol for amplification, library, library preparation, amplification
Funders Acknowledgements:
ÖAW
Grant ID: DI-2023-035
Abstract
A protocol for amplification and library preparation for Invertebrate metabarcoding on a MinION platform.
PCR Master mix



PCR Reaction


Library preparation for Sequncing on Oxford Nanopore MiniON