The ATP bioluminescence method can detect the ATP content in a sample by monitoring the biological luminescence reaction of a luciferase assay with a luminometer to detect the presence of microorganisms or other organic residues indirectly.Some studies demonstrated a good correlation between the ATP bioluminescence method and the colony counting method. However, due to the absence of uniform evaluation standards, most medical institutions rely on the reference values provided by the manufacturer of the testing detectors. And different detectors have different recommended values.Therefore, our study aimed to compare the ATP bioluminescence method with the colony counting method and the ATP bioluminescence method , also compare different ATP bioluminescence detector types, for monitoring the disinfection effect of environmental surfaces.