Mar 23, 2023

Public workspacePaintbrush method for C. elegans handling for microinjections

DOI
https://dx.doi.org/10.17504/protocols.io.5qpvor1r9v4o/v1
  • Theresa V Gibney1,
  • Ariel Pani1
  • 1University of Virginia, Charlottesville
Ariel M Pani
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DOI: https://dx.doi.org/10.17504/protocols.io.5qpvor1r9v4o/v1
Protocol Citation: Theresa V Gibney, Ariel Pani 2023. Paintbrush method for C. elegans handling for microinjections. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvor1r9v4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 19, 2023
Last Modified: March 23, 2023
Protocol Integer ID: 79040
Keywords: microinjection process, worm survival, microinjection, likelihood of worm survival, paintbrush method, paintbrush
Funders Acknowledgements:
NIH
Grant ID: R35GM142880
Abstract
See PDF attachment for protocol and links to videos.

This protocol describes a method for using a paintbrush to move C. elegans throughout the microinjection process. This method accelerates the microinjection process compared to traditional methods and improves the likelihood of worm survival.
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