Mar 01, 2022
Overall protocol for top-down LC-MS/MS of human spleen tissue
- Jeannie Camarillo1,
- Bryon Drown2,
- Neil Kelleher1
- 1Northwestern University;
- 2Purdue University
- Human BioMolecular Atlas Program (HuBMAP) Method Development Community
- Kelleher Research Group
Protocol Citation: Jeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down LC-MS/MS of human spleen tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5tgq6jw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58952
Keywords: ms data on human spleen tissue, ms of human spleen tissue overview description, human spleen tissue overview description, human spleen tissue, ms data, m
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human spleen tissue
Troubleshooting
Tissue Collection
Spleen tissue was provided by University of Florida TMC. Tissue was collected and prepared according to the following:
Sample Preparation
Perform LC based Proteomics on adjacent tissue sections:
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Data Acquisition
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Data Analysis
Protocol
CREATED BY
Kelleher KRG KRG Research Group