Mar 02, 2022
Overall protocol for top-down LC-MS/MS of human small intestine tissue
- Jeannie Camarillo1,
- Bryon Drown2,
- Neil Kelleher1
- 1Northwestern University;
- 2Purdue University
- Human BioMolecular Atlas Program (HuBMAP) Method Development Community
- Kelleher Research Group
Protocol Citation: Jeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down LC-MS/MS of human small intestine tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5tbq6in
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 02, 2022
Last Modified: March 02, 2022
Protocol Integer ID: 58947
Keywords: ms data on human small intestine tissue, ms of human small intestine tissue overview description, human small intestine tissue overview description, human small intestine tissue, ms data, m
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human small intestine tissue
Tissue Collection
Small intestine tissue was provided by Stanford University TMC. Tissue was collected according to the following:
Sample Preparation
Perform LC based Proteomics on adjacent tissue sections:
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Data Acquisition
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Sample Preparation
Protocol
CREATED BY
Kelleher KRG KRG Research Group