Mar 01, 2022
Overall protocol for top-down LC-MS/MS of human lung tissue
- Jeannie Camarillo1,
- Bryon Drown2,
- Neil Kelleher1
- 1Northwestern University;
- 2Purdue University
- Human BioMolecular Atlas Program (HuBMAP) Method Development Community
- Kelleher Research Group
Protocol Citation: Jeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down LC-MS/MS of human lung tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5sbq6an
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58915
Keywords: ms data on human lung tissue, ms of human lung tissue overview description, human lung tissue overview description, human lung tissue, human lung, ms data, m
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human lung tissue
Troubleshooting
Tissue Collection
Lung tissue was provided by University of Rochester Medical Center. Tissue was collected and prepared according to the following:
Protocol
CREATED BY
Gloria S Pryhuber
Sample Preparation
Perform LC based Proteomics on adjacent tissue sections:
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Data Acquisition
Protocol
CREATED BY
Kelleher KRG KRG Research Group
Sample Preparation
Protocol
CREATED BY
Kelleher KRG KRG Research Group