Mar 02, 2022

Public workspaceOverall protocol for top-down CZE-MS/MS of human spleen tissue

  • Jeannie Camarillo1,
  • Bryon Drown2,
  • Neil Kelleher1
  • 1Northwestern University;
  • 2Purdue University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
  • Kelleher Research Group
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Protocol CitationJeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down CZE-MS/MS of human spleen tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5tiq6ke
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 02, 2022
Last Modified: March 02, 2022
Protocol Integer ID: 58954
Keywords: ms data on human spleen tissue, ms of human spleen tissue overview description, human spleen tissue overview description, human spleen tissue, ms data, m, cze, tissue
Abstract
Overview description of the process for acquiring top-down CZE-MS/MS data on human spleen tissue
Tissue Collection
Spleen tissue was provided by University of Florida TMC. Tissue was collected and prepared according to the following:


Protocol
CREATED BY
Marda Jorgensen


Sample Preparation

Protocol
CREATED BY
Kelleher KRG KRG Research Group

Data Acquisition

Protocol
CREATED BY
Kelleher KRG KRG Research Group

Data Analysis

Protocol
CREATED BY
Kelleher KRG KRG Research Group