Mar 01, 2022

Public workspaceOverall protocol for top-down CZE-MS/MS of human small intestine tissue

  • Jeannie Camarillo1,
  • Bryon Drown2,
  • Neil Kelleher1
  • 1Northwestern University;
  • 2Purdue University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
  • Kelleher Research Group
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Protocol CitationJeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down CZE-MS/MS of human small intestine tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5tdq6i6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58949
Keywords: ms data on human small intestine tissue, ms of human small intestine tissue overview description, capillary zone electrophoresis m, human small intestine tissue overview description, human small intestine tissue, electrophoresi, ms data, m
Abstract
Overview description of the process for acquiring top-down capillary zone electrophoresis MS/MS data on human small intestine tissue
Troubleshooting
Tissue Collection
Small intestine tissue was provided by Stanford University TMC. Tissue was collected according to the following:

Sample Preparation

Protocol
CREATED BY
Kelleher KRG KRG Research Group

Data Acquisition

Protocol
CREATED BY
Kelleher KRG KRG Research Group

Data Analysis

Protocol
CREATED BY
Kelleher KRG KRG Research Group