This protocol is for over-agar antibiotic plating. To see the full abstract and additional resources, visit https:\/\/www.addgene.org\/protocols\/over-agar-antibiotic-plating\/Sample Data: Selection of E.coli on LB-agar using different concentrations of carbenicillin plated over-agar.Control Plate with No CarbenicillinPlate shows a lawn of E. coli and no selection.150 \u00b5L of 0.1 mg\/mL Carbenicillin plated over-agarPlate shows a lawn of E. coli and no apparent selection.150 \u00b5L of 1 mg\/mL Carbenicillin plated over-agarPlate shows several individual colonies and effective selection.150 \u00b5L of 2 mg\/mL Carbenicillin plated over-agarPlate shows less individual colonies than the 1 mg\/mL plate and effective selection.150 \u00b5L of 4 mg\/mL Carbenicillin plated over-agarPlate shows several individual colonies with smaller size than the 1 mg\/mL and 2 mg\/mL plates and effective selection.Selection Curve of Transformed E. coli after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin stock used (150 \u00b5L per plate). Please note we have found that there is generally a broad range of antibiotic concentrations that will work for this assay, and the above result represents a single experiment. For publishable data, the experiment would need to be repeated to account for variability.