Oct 03, 2023
OSU TriState SenNet Normal Donor Lung Acceptance Criteria
- Sean D. Stacey1,
- Brenda F. Reader1,
- Lorena Rosas2,
- Victor Peters2,
- Ana L. Mora2,
- mauricio.rojas 2
- 1Comprehensive Transplant Center, Wexner Medical Center, Ohio State University, Columbus, Ohio, USA.;
- 2Division of Pulmonary, Critical Care and Sleep Medicine, College of Medicine, Ohio State University, Columbus, Ohio, USA.
- TriState SenNet
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, mauricio.rojas 2023. OSU TriState SenNet Normal Donor Lung Acceptance Criteria. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1qzjkgr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 03, 2023
Last Modified: October 03, 2023
Protocol Integer ID: 88721
Keywords: biospecimen, Human lung tissue, normal donor, TriState SenNET, osu tristate sennet normal donor lung acceptance criteria, inclusion of human lung specimen, ctc human tissue biorepository, human lung specimen, comprehensive transplant center human tissue biorepository, tristate sennet biospecimen core, donor sample, tristate sennet biospecimen core at the ohio state, human tissue biorepository, part of the cellular senescence network program, cellular senescence network program, organ donor, routine laboratory specimen processing guideline, highest biospecimen quality, lung, tissue collection, comprehensive transplant center
Funders Acknowledgements:
TriState SenNET (Lung and Heart) Tissue Map and Atlas consortium - NIH
Grant ID: U54AG075931
Lifeline of Ohio and the generous donation from donors and their families
Abstract
This protocol describes the criteria required for inclusion of human lung specimen from healthy “normal” organ donors in the Comprehensive Transplant Center Human Tissue Biorepository and TriState SenNet Biospecimen Core at The Ohio State
University as part of the Cellular Senescence Network Program (SenNet). This protocol is based on the CTC Human Tissue Biorepository's standard operating procedure established for tissue collection for explant and donor samples. We follow
routine laboratory specimen processing guidelines to ensure the highest biospecimen quality.
Inclusion Criteria
Donor is consented for research by Lifeline of Ohio or registered organ donor, 18-80 years of age, negative for HCV, HBV, HIV, and no history of latent tuberculosis or active COVID-19
infection. Depending on the investigators’ request we remove specimens from upper, lower, or both lobes of a single lung.
When processing “normal” donor lung tissue, we choose samples from the healthiest section of the lung. We refer to the CT scan or chest x-ray to locate these regions.
For “normal” donor lungs, we look for the following characteristics:
1. Tissue is spongy.
2. Tissue is pink in color and color is uniform.
3. Absence of abscesses, nodules, or emphysematous presentations.
Exclusion Criteria
Donor is 17 years of age or younger, HBV, HCV, or HIV positive, has a latent
tuberculosis infection or active COVID-19 infection.