Jun 06, 2024
OSU TriState SenNet Normal Donor Heart Acceptance Criteria
- Sean D. Stacey1,
- Brenda F. Reader1,
- Lorena Rosas2,
- Victor Peters2,
- Ana L. Mora2,
- Mauricio Rojas2
- 1Comprehensive Transplant Center, Wexner Medical Center, Ohio State University, Columbus, Ohio, USA;
- 2Division of Pulmonary, Critical Care and Sleep Medicine, College of Medicine, Ohio State University, Columbus, Ohio, USA
- TriState SenNet
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, Mauricio Rojas 2024. OSU TriState SenNet Normal Donor Heart Acceptance Criteria. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l62255gqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 06, 2024
Last Modified: June 06, 2024
Protocol Integer ID: 101300
Keywords: biospecimen, human heart tissue, normal donor, TriState SenNET, osu tristate sennet normal donor heart acceptance criteria, normal donor heart, comprehensive transplant center human tissue biorepository, ctc human tissue biorepository, tristate sennet biospecimen core, human tissue biorepository, donor sample, inclusion of human heart, part of the cellular senescence network program, organ donor, cellular senescence network program, comprehensive transplant center, highest biospecimen quality, human heart, tissue collection, routine laboratory specimen processing guideline
Funders Acknowledgements:
TriState SenNET (Lung and Heart) Tissue Map and Atlas consortium
Grant ID: U54AG075931
Lifeline of Ohio and the generous donation from donors and their families.
Grant ID: N/A
Disclaimer
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Abstract
This protocol describes the criteria required for the inclusion of human heart specimens from healthy “normal” organ donors in the Comprehensive Transplant Center Human Tissue Biorepository and TriState SenNet Biospecimen Core at The Ohio State University as part of the Cellular Senescence Network Program (SenNet). This protocol is based on the CTC Human Tissue Biorepository's standard operating procedure established for tissue collection for explant and donor samples. We follow routine laboratory specimen processing guidelines to ensure the highest biospecimen quality.
Inclusion Criteria
The donor is authorized for research by Lifeline of Ohio, Central Ohio’s organ procurement agency, 18-80 years of age, negative for HCV, HBV, HIV, and has no active COVID-19 infection. The whole heart is procured by Lifeline of Ohio and the biorepository then provides the de-identified heart to the researcher. When processing “normal” donor heart tissue, samples are chosen from the healthiest section of the five target regions of the heart: septum, left and right ventricles, and left and right atrium. The CT scans or chest X-rays are referenced to locate any diseased portions of these regions or to assess the overall health of the donor heart.
To be considered a “normal” donor heart, the donor has no known history of heart failure, no current infection, and limited inflammation.
Exclusion Criteria
The donor is 17 years of age or younger, HBV, HCV, or HIV positive, or has an active COVID-19 infection.