Jan 11, 2023
OSU TriState SenNet lung explant from IPF patients acceptance criteria
- Sean D. Stacey1,
- Brenda F. Reader1,
- Lorena Rosas2,
- Victor Peters2,
- Ana L. Mora2,
- Mauricio Rojas2
- 1Comprehensive Transplant Center, Wexner Medical Center, Ohio State University, Columbus, Ohio, USA;
- 2Division of Pulmonary, Critical Care and Sleep Medicine, College of Medicine, Ohio State University, Columbus, Ohio, USA.
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, Mauricio Rojas 2023. OSU TriState SenNet lung explant from IPF patients acceptance criteria. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpjyo1gzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 11, 2023
Last Modified: January 11, 2023
Protocol Integer ID: 75161
Keywords: osu tristate sennet lung explant, inclusion of human lung specimen explant, human lung specimen explant, idiopathic pulmonary fibrosi, ctc human tissue biorepository, patients in comprehensive transplant center human tissue biorepository, comprehensive transplant center human tissue biorepository, ipf patients acceptance criteria, part of the cellular senescence network program, tristate sennet biospecimen core, diseased tissue, human tissue biorepository, donor sample, cellular senescence network program, comprehensive transplant center, tissue collection, ct scan
Funders Acknowledgements:
TriState SenNET (Lung and Heart) Tissue Map and Atlas Consortium - NIA
Grant ID: U54AG075931
Abstract
This protocol describes the criteria required for inclusion of human lung specimen explants from idiopathic pulmonary fibrosis (IPF) diagnosed patients in Comprehensive Transplant Center Human Tissue Biorepository and TriState SenNet Biospecimen Core at The Ohio State University as part of the Cellular Senescence Network Program (SenNet). These criteria are based off of the CTC Human Tissue Biorepository SOP:009 Tissue Collection for Explant and Donor Samples. When processing explanted, diseased tissue, look for the most representative of that disease. Refer to the CT scan or Chest X-ray on the committee review to locate most diseased areas.
Troubleshooting
Inclusion Criteria
Transplant and surgical: 18-80 years of age, patient at OSUWMC, referred to OSUWMC for transplantation or has already undergone transplantation, able to understand and sign the TTCP Informed Consent and Research Authorization form, negative for HCV, HBV, and HIV, and has a pathology-confirmed diagnosis of IPF.Due to the nature of IPF pathogenesis, we remove specimen from both upper and lower lobes of a single lung. When processing explanted, diseased tissue, we choose samples from section of the lung that is the most representative of that disease. We refer to the CT scan or chest x-ray to locate these areas.
In the case of IPF, we look for the following characteristics:
a)tissue is fibrotic,
b)tissue shows thickening and scarring,
c)tissue feels hard and stiff to the touch.
Exclusion Criteria
17 years of age and younger and unable to understand and sign the TTCP Informed Consent and Research Authorization form directly.