Jun 19, 2026

OSMAC Culture procedure for fungi

DOI
https://dx.doi.org/10.17504/protocols.io.kxygxreo4g8j/v1
  • Yoan Ferandin1,
  • Clémence ROHÉE1,
  • Alice M.S. RODRIGUES1,
  • Emeline HOUEL1,
  • Didier Stien1
  • 1Sorbonne Université, CNRS, UPVD, UMR8176, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, 66650 Banyuls-sur-Mer, France
  • EUREMAP PROTOCOLS COMMUNITY
  • EUREMAP_SU_LBBM
Clémence ROHÉE
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DOI: https://dx.doi.org/10.17504/protocols.io.kxygxreo4g8j/v1
Protocol Citation: Yoan Ferandin, Clémence ROHÉE, Alice M.S. RODRIGUES, Emeline HOUEL, Didier Stien 2026. OSMAC Culture procedure for fungi. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygxreo4g8j/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 18, 2026
Last Modified: June 19, 2026
Protocol  Integer ID: 319408
Keywords: osmac culture procedure for fungi, osmac culture procedure, medium fungal culture, osmac method, fungi, biological activity, diverse extract
Funders Acknowledgements:
EUROPEAN UNION
Grant ID: INFRA-2023-DEV-01-04
Abstract
This protocol describes how to obtain diverse extracts from solid-medium fungal cultures and enhance their biological activity using the OSMAC method.
Materials
Glass bottles
Powders for each medium
14.5 cm diameter Petri dishes
pH meter
1 mL pipette
filter tips
static incubator
Safety warnings
Use lab coat and microbiological safety cabinet.
Preparation of culture media and inoculation
About 50 ml of medium were poured in large petri dishes.
All media pH were adjusted to 7.2-7.4.
Solid media were inoculated with 3 ml of a fresh (5-10 days) liquid culture (YMG medium containing 10 g Malt extract, 4 g glucose, 1 g yeast extract 17 g/l SS).
Incubation at 25°C for 2-4 weeks (depending on fungi growth on medium).
Medium 1: MGY SS; per liter: Malt 10 g, Yeast Extract 1 g, Glucose 4 g, Sea salt 17 g, Agar agar 12 g.
Medium 2: PDM SS; per liter: potato dextrose medium 26.5 g, Sea salt 16 g, Agar agar 12 g.
Medium 3: GPY SS; per liter: Glucose 1 g, yeast extract 0.1 g, Peptone 0.5 g, Sea salt 16 g, Agar agar 12 g.
Medium 4: Sucrose Yeast Medium SYM SS; per liter: Sucrose 20 g, yeast extract 10 g, Sea Salt 16 g, Agar agar 12 g.
Medium 5: malt medium with added sea salts; per liter: malt extract 4 g, sea salts 40 g, Agar agar 12 g.
Medium 6: Malt Extract seawater; per liter: malt extract 20 g, glucose 20 g, peptone 2 g, Agar agar 12 g.
Medium 8: Czapek; per liter: Czapek Dox 33.4 g, Sea Salt 16 g, Agar agar 12 g.
Medium 9: MGY without SS; per liter: Malt 10 g, Yeast Extract 1 g, Glucose 4 g, Agar agar 12 g.
Medium 10: PDM without SS; per liter: PDB 26.5 g, Agar agar 12 g.
Medium 11: 1gGPY without SS; per liter: Glucose 1 g, yeast extract 0.1 g, Peptone 0.5 g, Agar agar 12 g.
Medium 12: M without SS; per liter: malt extract 20 g, glucose 20 g, peptone 2 g, Agar agar 12 g.
Medium 13: SYM without SS; per liter: Sucrose 5 g, yeast extract 2.5 g, Agar agar 3 g.
Medium 14: KZAPEK without SS; per liter: Milieu Czapek Dox 8.35 g, Agar agar 3 g.