Mar 22, 2024

Public workspaceOral Fat Tolerance Test (OFTT) in Human

DOI
dx.doi.org/10.17504/protocols.io.36wgq3dy5lk5/v1
Oral Fat Tolerance Test (OFTT) in Human
  • 1Universidade Federal Fluminense, Nova Friburgo, RJ, Brazil, https://orcid.org/0000-0002-6528-3932;
  • 2Universidade Federal Fluminense, Nova Friburgo, RJ, Brazil, https://orcid.org/0000-0003-4177-9383;
  • 3Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil, http://orcid.org/0000-0001-8138-7734;
  • 4Universidade Federal do Rio de Janeiro, Macaé, RJ, Brazil, https://orcid.org/0000-0001-8792-7682;
  • 5Federal Fluminense University
Caroline Fernandes-Santos
Open access
DOI: dx.doi.org/10.17504/protocols.io.36wgq3dy5lk5/v1
Protocol Citation: Francine dos Santos-Macedo, Dilliane da Paixão Rodrigues Almeida, Bianca Martins Gregório, Fernanda Amorim de Morais Nascimento Braga, Caroline Fernandes-Santos 2024. Oral Fat Tolerance Test (OFTT) in Human. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq3dy5lk5/v1
Manuscript citation:
Santos-Macedo et al., 2024. A Practical Standardized Fat Challenge for the Oral Fat Tolerance Test (OFTT) in Men and Women. Vol.67: e24220988, 2024. https://doi.org/10.1590/1678-4324-2024220988
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 18, 2023
Last Modified: March 22, 2024
Protocol Integer ID: 87890
Keywords: OFTT, Postprandial lipemia, Triglyceride, Hypertriglyceridemia, Human, Fat meal
Disclaimer
The authors declare that there are no conflicts of interest associated with this research study.
Abstract
Following the consumption of a standardized high-fat meal, the oral fat tolerance test (OFTT) assesses the postprandial lipemic (PPL) response of blood triglycerides (TG). Blood samples are obtained four hours after meal ingestion, with a targeted TG level of ≤ 220 mg/dL considered within the normal range. An altered PPL response is defined by TG levels exceeding 220 mg/dL. The PPL is influenced by various factors, such as the quality and type of foods ingested in the diet, physical exercise, body metabolism, and genetics. The PPL response may predict the development of Atherosclerotic Cardiovascular Disease, thus serving as an early marker of the atherosclerotic process associated with metabolic disorders.
Materials
OFTT meal

  1. Kitchen blender
  2. Milk cream (Nestlé)
  3. Cocoa powder (Mãe Terra, São Paulo, Brazil)
  4. Dry nonfat milk (Glória, São Paulo, Brazil)
  5. Sugar
  6. Cold water
  7. Disposable plastic cup (500 mL)
  8. Straw (diameter 21 cm x 10 mm)
Use the table below to mix the ingredients:

Captura de tela 2023-09-18 081932.png


Before start
Depending on the blender available, preparing the volume of 4 meals at once is more practical because there are losses on the blender's wall. Participants must fast for 8 to 10 hours before blood collection. They should avoid consuming fatty foods and engaging in physical activity in the 72 hours before the test.
OFTT meal preparation (milkshake)
OFTT meal preparation (milkshake)
15m
Identify the cup with the participant's name.
Weigh the empty cup and record it (W1).
1m
Weight the ingredients
5m
Add the ingredients to the blender and mix.
2m
Distribute the milkshake into plastic cups.
Weigh the whole cup and record it (W2).
Place the lid and straw.
Note
The meal should be served immediately after preparation. If it sits on the countertop too long (more than 30 min), it may separate into layers, and the visual aspect reduces participant acceptability.


Expected result
The OFTT meal should resemble a cold chocolate milkshake. This appearance increases the milkshake acceptability by the research participant.


Oral Fat Tolerance Test (OFTT)
Oral Fat Tolerance Test (OFTT)
4h 30m
Collect venous blood from the arm using a 5 mL syringe and reserve it in a serum separator tube.
Offer the test meal to the study participant.
Monitor the time it takes for the participant to consume the milkshake. They should consume as much as possible.
After consumption is complete, weigh the cup (W3).
Collect a new venous blood sample from the arm 4 hours after the start of meal ingestion.
Calculating milkshake intake
Calculating milkshake intake
Apply the following formula:

Serum triglyceride assay
Serum triglyceride assay
Centrifuge the blood at 2,000 x g for 10 minutes.
Transfer the serum to 2.0 mL microtubes.
Conduct the biochemical assay following the manufacturer's instructions.
Protocol references
Kolovou, G. D., Watts, G. F., Mikhailidis, D. P., Pérez-Martínez, P., Mora, S., Bilianou, H., Panotopoulos, G., Katsiki, N., Ooi, T. C., Lopez-Miranda, J., Tybjærg-Hansen, A., Tentolouris, N., & Nordestgaard, B. G. (2019). Postprandial Hypertriglyceridaemia Revisited in the Era of Non-fasting Lipid Profiles: Executive Summary of a 2019 Expert Panel Statement. Current vascular pharmacology, 17(5), 538–540. https://doi.org/10.2174/1570161117999190517115432

Kolovou, G. D., Mikhailidis, D. P., Kovar, J., Lairon, D., Nordestgaard, B. G., Ooi, T. C., Perez-Martinez, P., Bilianou, H., Anagnostopoulou, K., & Panotopoulos, G. (2011). Assessment and clinical relevance of non-fasting and postprandial triglycerides: an expert panel statement. Current vascular pharmacology, 9(3), 258–270. https://doi.org/10.2174/157016111795495549