Dec 22, 2025
Opto-Open Field
- Asa Mackenzie1
- 1Lund University
Protocol Citation: Asa Mackenzie 2025. Opto-Open Field. protocols.io https://dx.doi.org/10.17504/protocols.io.261ge164yv47/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 22, 2025
Last Modified: December 23, 2025
Protocol Integer ID: 235678
Keywords: ASAPCRN, open field opto, open field from serra et al, open field, opto, serra et al
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020600
Abstract
Opto-Open Field from Serra et al 2023
Troubleshooting
Mice were individually placed in neutral cages for 3 min in order to recover after connecting the optic cables.
Mice were subsequently
placed in the central zone of the open field arena and allowed to freely explore it for 5 min before starting the test.
The open-field
chamber consisted in a 50 cm, squared, transparent, plastic arena with a white floor.
The Opto-Open Field test consisted of a
20-min session divided into 5-min alternating time intervals (OFF-ON-OFF-ON).
Each 5-min interval was then divided into two
2.5-min intervals for analysis.
The patch cable was connected to a rotary joint, which was attached on the other end to a laser
that was controlled by an Arduino Uno card (Arduino).
During the light-ON trials, blue light was delivered according to the behavioral
stimulation protocol (20Hz, 5ms, 5mW).
Self-grooming and escape behaviors were manually recorded by an experimenter blind to
the experimental groups using the EthoVision XT 14.0 tracking software (Noldus Information Technology, The Netherlands).