Dec 22, 2025

Public workspaceOpto-negative reinforcement paradigm (Opto-NR)

DOI
https://dx.doi.org/10.17504/protocols.io.yxmvm17nnv3p/v1
  • Asa Mackenzie1
  • 1Lund University
amanda schneeweis
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DOI: https://dx.doi.org/10.17504/protocols.io.yxmvm17nnv3p/v1
Protocol Citation: Asa Mackenzie 2025. Opto-negative reinforcement paradigm (Opto-NR). protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm17nnv3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 22, 2025
Last Modified: December 23, 2025
Protocol Integer ID: 235684
Keywords: ASAPCRN, negative reinforcement paradigm, opto
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020600
Abstract
Opto-negative reinforcement paradigm (Opto-NR) in Serra et al 2023
Troubleshooting
Opto-negative reinforcement paradigm (Opto-NR)
Mice response was assessed by using operant conditioning boxes equipped with two nose-poke devices (MED-PC, Med Associates inc, Fairfax, USA) and laser for optogenetic stimulation
One device was defined as active, which when activated terminates the optogenetic stimulation schedule, while the other one was demarcated as inactive, which had no control over the laser.
The presentation of the optogenetic stimulations (10 s) were alternated with variable-time intervals, when no stimulation was applied.
An active nose-poke (FR1) at any point during this pre-stimulation interval or during the stimulation epoch itself terminates the optogenetic stimulation schedule and produces a visible safety signal (house light ON) that marks a period of safety.