Optical mapping technologies assist the assembly of large and complex genomes. Although protocols for preparing this kind of samples are now conveniently available in kits, some plants won't respond to the standardized conditions or the researcher might not have access to it. Here I provide a draft protocol I have been working on for kiwifruit, with limited success. The nuclear genomic DNA produced is ~ 1 Mbp long but it has not passed the service provider QC step. I would be very happy to hear from the ResearchGate and Protocos.io communities regarding any interest in collaborating to improve this method and eventually make it available to a wider audience.