Apr 03, 2024
Open-source tools for Spatial Tissue Exploration Program (STEP) PhenoCycler-Fusion image analysis.
This protocol is a draft, published without a DOI.
- Daniel H Geschwind1,
- George Chen1
- 1UCLA
External link: https://www.akoyabio.com/akoya-academy/
Protocol Citation: Daniel H Geschwind, George Chen 2024. Open-source tools for Spatial Tissue Exploration Program (STEP) PhenoCycler-Fusion image analysis.. protocols.io https://dx.doi.org/
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 03, 2024
Last Modified: April 03, 2024
Protocol Integer ID: 97729
Keywords: Spatial Tissue Exploration Program (STEP) , PhenoCycler, Akoya Biosciences, source tools for spatial tissue exploration program, spatial tissue exploration program, ffpe tissue section, phenocycler user guide, link to phenocycler user guide, fusion image analysis, phenocycler, image analysis
Disclaimer
These are protocols created by Akoya Biosciences that we use. We do not claim any IP for these protocols.
Abstract
Link to PhenoCycler User Guide to allow for multi-plex spatial proteomic detection on fresh frozen and FFPE tissue sections plus image analysis.
STEP protocol for PhenoCycler coverslip preparation
STEP protocol for PhenoCycler coverslip preparation can be found here:
PhenoCycler Workflow STEP Sample Preparation.pdf144.4KB
PhenoCycler Workflow STEP Sample Preparation.pdf144.4KB This protocol is working for us.
PhenoCycler Imaging Analysis protocol
PhenoCycler open source analysis.pdf5.7MB Imaging Analysis protocol can be found here:
This protocol is working for us.
Protocol references
More references available here: https://www.akoyabio.com/akoya-academy/