Sep 08, 2020
Open Isothermal Platform Protocal
- Andrew Buultjens1,
- Koen Vandelannoote2,
- Tim Stinear1
- 1The University of Melbourne;
- 2Institut Pasteur du Cambodge
- XPRIZE Rapid Covid Testing
- Open-Isothermal-Platforms Dx
Protocol Citation: Andrew Buultjens, Koen Vandelannoote, Tim Stinear 2020. Open Isothermal Platform Protocal. protocols.io https://dx.doi.org/10.17504/protocols.io.bk3nkyme
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 08, 2020
Last Modified: September 08, 2020
Protocol Integer ID: 41806
Extract RNA as per dx.doi.org/10.17504/protocols.io.bkynkxve
Transfer 5ml of extracted RNA to pre-aliquoted LAMP mix. Mix by pipetting up and down 5 times.
Add 5ml of H2O in negative control well 7.
Add 5ml of positive control in well 8.
Cap all wells
Place strips in FABL-8
Run FABL-8 as per manual
LAMP reaction (can refer to N1-STOP-LAMP paper)
Single reaction setup:
| Reagent | Volume (ml) | |
| Optigene Master Mix | 15 | |
| N1 primer | 5 | |
| Extracted RNA | 5 | |
| Total | 25 |
To reduce pipetting error, Master Mix and N1 primer are premixed according to the number of reactions desired (allow for pipetting error by preparing enough for ~1.1x the number of reactions, rounded to the nearest whole number); 20ml of this mixture is then aliquoted per reaction.