Feb 09, 2024

Open Field

DOI
https://dx.doi.org/10.17504/protocols.io.kxygx3dbog8j/v1
  • 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Jacquelyn Haytayan
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DOI: https://dx.doi.org/10.17504/protocols.io.kxygx3dbog8j/v1
Protocol Citation: daniel.dautan , Per Svenningsson 2024. Open Field. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx3dbog8j/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 09, 2024
Last Modified: September 24, 2024
Protocol  Integer ID: 94932
Keywords: ASAPCRN, behavior, motor, general locomotion in mice, mice, general locomotion, open field behavioral
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Behavioral assay for general locomotion in mice.
Materials
  • Open field arenas: 40 x 40 dark box
  • Recording camera
  • Ethovision XT v11
Habituate naive mice to the room for 01:00:00 .
1h
Transfer each mouse to a custom 40 cm x 40 cm dark box, with a clear floor and a light suspended on the center of the apparatus.
Test duration was set at 00:20:00 .

20m
After each test and between each group, clean apparatus thoroughly with 50% ethanol followed by water.
Analysis:
  • Center of open field is defined by the space located at least 5 cm from any given wall.
  • Time in the center, distance travelled, and average speed is obtained by the built-in functions of the tracking software Ethovision XT11.