Dec 10, 2024
Version 1
OGI Bio Bung Cleaning V.1
- 1OGI Bio
- OGI Bio
Protocol Citation: Jack Hocking 2024. OGI Bio Bung Cleaning . protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmeq29g3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 08, 2024
Last Modified: December 10, 2024
Protocol Integer ID: 104960
Disclaimer
OGI Bio does not accept responsibility for damage incurred to the OGI BioReactor system or its additional modules or accessories caused by cleaning in any way not specified within these protocols.
Abstract
The protocol for the cleaning and sterilization of your OGI BioReactor silicon bungs.
Guidelines
Bungs should be cleaned immediately after an experiment to prevent fouling in the needles due to dry culture.
Take care not to bend the Media/Waste bung needles, as they are carefully designed to fit standard 500 mL Duran bottles.
Materials
OGI Bio Bungs:
Equipment
Culture Flask Bung
NAME
Accessory
TYPE
ŌGi Bio
BRAND
P500032
SKU
LINK
Equipment
Media/Waste Flask Bung
NAME
Accessory
TYPE
ŌGI Bio
BRAND
P500033
SKU
LINK
Cleaning Matierals:
70% Ethanol Contributed by users
Safety warnings
Take care when handling the bungs as, while care is taken to ensure no cutting edges are left, the needles can be sharp.
Before start
Ensure you have familiarised yourself with the guidelines and warnings for this protocol.
Cleaning
Cleaning
As soon after running an experiment as possible, using a small piece of 1 mm ID tubing, flush both Source (S) and Drain (D) needles with ethanol to prevent debris buildup.
If using, flush the needle of the Waste flask bung with ethanol.
Sterilization
Sterilization
As the needles are sharp, double-bag the bungs in paper autoclave bags.
Equipment
Steribags Head Seal Closure Pouches
NAME
Autoclave Bag
TYPE
Synergy Healthcare
BRAND
15671944
SKU
LINK
Autoclave at the preferred temperature. The bungs are temperature-safe up to 250°C.
Bungs should not be removed from their autoclave bags unless under sterile conditions.