This protocol is a continuation of the previous protocol (dx.doi.org/10.17504/protocols.io.m4yc8xw) for viral metagenomic researches with the tissues of Molluscs, such as abalone, oyster, clam, scallop and so on. This maybe also applicable for other animals, such as shrimp and fish, based on the principle of the protocol. But we havn't verified this idea yet.