Nov 20, 2020

Public workspaceNuclei preparation from human lung using grinding for snRNA-seq

DOI
dx.doi.org/10.17504/protocols.io.bprdmm26
  • 1UCSD
Sebastian Preissl
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DOI: dx.doi.org/10.17504/protocols.io.bprdmm26
Protocol Citation: Center for Epigenomics UCSD 2020. Nuclei preparation from human lung using grinding for snRNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.bprdmm26
Manuscript citation:
Wang, A. et al. Single cell multiomic profiling of human lung reveals cell type-specific and age-dynamic control of SARS-CoV2 host genes. Elife 9, doi:10.7554/eLife.62522 (2020).
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 16, 2020
Last Modified: November 20, 2020
Protocol Integer ID: 44549
Keywords: lung, nuclei preparation, single nucleus RNA-seq
Abstract
Protocol describing nuclei isolation from frozen tissue including lung using grinding for droplet-based single nucleus RNA-seq.
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