Nov 19, 2020

Public workspaceNuclei preparation from human lung using douncing for snRNA-seq

DOI
dx.doi.org/10.17504/protocols.io.bnvkme4w
  • 1UCSD
Sebastian Preissl
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DOI: dx.doi.org/10.17504/protocols.io.bnvkme4w
Protocol Citation: Center for Epigenomics UCSD 2020. Nuclei preparation from human lung using douncing for snRNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.bnvkme4w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 23, 2020
Last Modified: November 19, 2020
Protocol Integer ID: 43660
Keywords: nucleus preparation, frozen tissue, human lung, single nucleus RNA-seq
Abstract
Protocol describing nuclei isolation from frozen tissue including lung using douncing for droplet-based single nucleus RNA-seq.
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