Nov 19, 2020

Public workspaceNuclei isolation from human lung using grinding for snATAC-seq

DOI
dx.doi.org/10.17504/protocols.io.bnvmme46
  • 1UCSD
Sebastian Preissl
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DOI: dx.doi.org/10.17504/protocols.io.bnvmme46
Protocol Citation: Center for Epigenomics UCSD 2020. Nuclei isolation from human lung using grinding for snATAC-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.bnvmme46
Manuscript citation:
Wang, A. et al. Single cell multiomic profiling of human lung reveals cell type-specific and age-dynamic control of SARS-CoV2 host genes. Elife 9, doi:10.7554/eLife.62522 (2020).
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 23, 2020
Last Modified: November 19, 2020
Protocol Integer ID: 43661
Keywords: nucleus preparation, human lung, single cell ATAC-seq, frozen tissue,
Abstract
Protocol describing nuclei isolation from frozen tissue including lung using grinding for single nucleus ATAC-seq.
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